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s protein cdna construct  (Sino Biological)


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    Structured Review

    Sino Biological s protein cdna construct
    Fragment of the multiple alignments of SARS-CoV-2 <t>S</t> <t>protein</t> RNAs: Sequences surrounding the CCTCGGCGGGCA insertion in the <t>SARS-CoV-2</t> <t>sequence</t> (GenBank entry NC_045512.2, the SARS-CoV-2 reference sequence). MN996532.1 is the closest bat homolog RaTG13; MG772934.1, MG772933.1, and KT444582.1 are more distantly related bat homologs. Novel out-of-frame stop codons in the human SARS-CoV-2 are italicized.
    S Protein Cdna Construct, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s+protein+cdna+construct/pmc08235447-88-24-34?v=Sino+Biological
    Average 94 stars, based on 90 article reviews
    s protein cdna construct - by Bioz Stars, 2026-08
    94/100 stars

    Images

    1) Product Images from "The Functional Consequences of the Novel Ribosomal Pausing Site in SARS-CoV-2 Spike Glycoprotein RNA"

    Article Title: The Functional Consequences of the Novel Ribosomal Pausing Site in SARS-CoV-2 Spike Glycoprotein RNA

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22126490

    Fragment of the multiple alignments of SARS-CoV-2 S protein RNAs: Sequences surrounding the CCTCGGCGGGCA insertion in the SARS-CoV-2 sequence (GenBank entry NC_045512.2, the SARS-CoV-2 reference sequence). MN996532.1 is the closest bat homolog RaTG13; MG772934.1, MG772933.1, and KT444582.1 are more distantly related bat homologs. Novel out-of-frame stop codons in the human SARS-CoV-2 are italicized.
    Figure Legend Snippet: Fragment of the multiple alignments of SARS-CoV-2 S protein RNAs: Sequences surrounding the CCTCGGCGGGCA insertion in the SARS-CoV-2 sequence (GenBank entry NC_045512.2, the SARS-CoV-2 reference sequence). MN996532.1 is the closest bat homolog RaTG13; MG772934.1, MG772933.1, and KT444582.1 are more distantly related bat homologs. Novel out-of-frame stop codons in the human SARS-CoV-2 are italicized.

    Techniques Used: Sequencing

    Expression of S protein in pseudovirions expressing original and codon-optimized S proteins. Pseudovirions were produced in LV production cells, Expi293 cells, and adherent HEK293T cells. Upper panels were imaged at normal gain, while lower panels were imaged at higher gain to visualize original S protein expression (S original). Red asterisk indicates spike protein band.
    Figure Legend Snippet: Expression of S protein in pseudovirions expressing original and codon-optimized S proteins. Pseudovirions were produced in LV production cells, Expi293 cells, and adherent HEK293T cells. Upper panels were imaged at normal gain, while lower panels were imaged at higher gain to visualize original S protein expression (S original). Red asterisk indicates spike protein band.

    Techniques Used: Expressing, Produced



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    Fragment of the multiple alignments of SARS-CoV-2 <t>S</t> <t>protein</t> RNAs: Sequences surrounding the CCTCGGCGGGCA insertion in the <t>SARS-CoV-2</t> <t>sequence</t> (GenBank entry NC_045512.2, the SARS-CoV-2 reference sequence). MN996532.1 is the closest bat homolog RaTG13; MG772934.1, MG772933.1, and KT444582.1 are more distantly related bat homologs. Novel out-of-frame stop codons in the human SARS-CoV-2 are italicized.
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    Image Search Results


    Fragment of the multiple alignments of SARS-CoV-2 S protein RNAs: Sequences surrounding the CCTCGGCGGGCA insertion in the SARS-CoV-2 sequence (GenBank entry NC_045512.2, the SARS-CoV-2 reference sequence). MN996532.1 is the closest bat homolog RaTG13; MG772934.1, MG772933.1, and KT444582.1 are more distantly related bat homologs. Novel out-of-frame stop codons in the human SARS-CoV-2 are italicized.

    Journal: International Journal of Molecular Sciences

    Article Title: The Functional Consequences of the Novel Ribosomal Pausing Site in SARS-CoV-2 Spike Glycoprotein RNA

    doi: 10.3390/ijms22126490

    Figure Lengend Snippet: Fragment of the multiple alignments of SARS-CoV-2 S protein RNAs: Sequences surrounding the CCTCGGCGGGCA insertion in the SARS-CoV-2 sequence (GenBank entry NC_045512.2, the SARS-CoV-2 reference sequence). MN996532.1 is the closest bat homolog RaTG13; MG772934.1, MG772933.1, and KT444582.1 are more distantly related bat homologs. Novel out-of-frame stop codons in the human SARS-CoV-2 are italicized.

    Article Snippet: Based on the bioinformatic analyses, and to compare the expression of the original sequence spike protein and codon-optimized spike protein, we obtained the codon-optimized S protein cDNA construct (CCAAGGAGGGCA furin site, pCMV-codon-optimized spike, VG40589-UT, Sino Biologicals) and the original sequence cDNA construct (CCTCGGCGGGCA furin site, pUNO1-SARS2-S, InvivoGen).

    Techniques: Sequencing

    Expression of S protein in pseudovirions expressing original and codon-optimized S proteins. Pseudovirions were produced in LV production cells, Expi293 cells, and adherent HEK293T cells. Upper panels were imaged at normal gain, while lower panels were imaged at higher gain to visualize original S protein expression (S original). Red asterisk indicates spike protein band.

    Journal: International Journal of Molecular Sciences

    Article Title: The Functional Consequences of the Novel Ribosomal Pausing Site in SARS-CoV-2 Spike Glycoprotein RNA

    doi: 10.3390/ijms22126490

    Figure Lengend Snippet: Expression of S protein in pseudovirions expressing original and codon-optimized S proteins. Pseudovirions were produced in LV production cells, Expi293 cells, and adherent HEK293T cells. Upper panels were imaged at normal gain, while lower panels were imaged at higher gain to visualize original S protein expression (S original). Red asterisk indicates spike protein band.

    Article Snippet: Based on the bioinformatic analyses, and to compare the expression of the original sequence spike protein and codon-optimized spike protein, we obtained the codon-optimized S protein cDNA construct (CCAAGGAGGGCA furin site, pCMV-codon-optimized spike, VG40589-UT, Sino Biologicals) and the original sequence cDNA construct (CCTCGGCGGGCA furin site, pUNO1-SARS2-S, InvivoGen).

    Techniques: Expressing, Produced